Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a mutant strain WX-02 DELTAbudC of Bacillus licheniformis with depleted budC gene that produces high yields of the D-2,3-butanediol isomer with high optimal purity | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | Bacillus licheniformis | - |
acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | Bacillus licheniformis WX-02 | - |
acetoin + NADH + H+ | - |
r | |
acetoin + NADH + H+ | Bacillus licheniformis | - |
(2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
acetoin + NADH + H+ | Bacillus licheniformis WX-02 | - |
(2R,3S)-butane-2,3-diol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus licheniformis WX-02 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | - |
Bacillus licheniformis | acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | - |
Bacillus licheniformis WX-02 | acetoin + NADH + H+ | - |
r | |
acetoin + NADH + H+ | - |
Bacillus licheniformis | (2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
acetoin + NADH + H+ | - |
Bacillus licheniformis WX-02 | (2R,3S)-butane-2,3-diol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
budC | - |
Bacillus licheniformis |
meso-2,3-butanediol dehydrogenase | - |
Bacillus licheniformis |
meso-acetoin reductase | - |
Bacillus licheniformis |
meso-BDH | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus licheniformis | |
NADH | - |
Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
malfunction | the budC knockout strain produces only the D-2,3-butanediol isomer with high yield and productivity. Deletion of budC gene causes a slight decrease (about 5-10%) in cell growth | Bacillus licheniformis |
physiological function | budC encodes the major meso-2,3-butanediol dehydrogenase catalyzing the reversible reaction from acetoin to meso-2,3-butanediol in Bacillus licheniformis | Bacillus licheniformis |